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Berlin 2008 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 9: POSTERS Single Molecules, Biopolymers, Membranes

CPP 9.15: Poster

Monday, February 25, 2008, 16:45–19:00, Poster A

High energy reflectivity studies of protein adsorption on functionalized surfaces — •Kaveh Shokuie1, Michael Paulus1, Florian Evers1, Federica Venturini2, Sebastian Schoeder2, and Metin Tolan11Experimentelle Physik Ia/ DELTA, Technische Universität Dortmund, Maria-Goeppert-Meyer Str. 2, 44221 Dortmund — 2European Synchrotron Radiation Facility, Grenoble, France

Adsorption of several proteins (Lysozyme, Hemoglobin, α-lactalbumin) on functionalized surfaces was investigated using high energy X-ray reflectivity. The main interest is the effect of different substrate surfaces on the protein adsorption behaviour (e.g. conformational changes of the protein). The applied technique, X-ray reflectivity, makes it possible to determine the sample’s density profile perpendicular to its surface with Angstrom resolution. The high energy reflectivities were taken at the ID15 beamline of the ESRF using 72.5 keV radiation. The high photon energy allows the in situ investigation of the protein adsorption at the solid-liquid interface.

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