Berlin 2008 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 9: POSTERS Single Molecules, Biopolymers, Membranes

CPP 9.16: Poster

Montag, 25. Februar 2008, 16:45–19:00, Poster A

Biofilm adsorption on tailored substrates: Ellipsometry, AFM, SPR and neutron scattering results — •Hendrik Hähl, Hubert Mantz, Yvonne Schmitt, and Karin Jacobs — Saarland University, Experimental Physics, D-66123 Saarbrücken, Germany

Whenever a protein solution is in contact with a material, the proteins will adsorb onto the surface. Everyday examples for this process are biofilms on teeth. This study aims to reveal the influence of surface properties on the adsorption process. Our results show that the adsorption kinetics of e.g. α-amylase is sensitive to the thickness of the oxide layer on top of the Si wafer. This is astonishing since the chemistry of the offered surfaces is identical, meaning that the short-range forces are not varied. Only the long-range forces are altered by a variation of the oxide layer thickness, and they play a central role for the adsorption kinetics. Theoretical models and simulations suggest that a variation of long-range forces can lead to conformational changes of the proteins during the adsorption. To test this hypothesis, experiments with atomic force microscopy (AFM), surface plasmon resonance (SPR) spectroscopy and ellipsometry as well as neutron reflectivity measurements have been performed.