Berlin 2008 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 9: POSTERS Single Molecules, Biopolymers, Membranes

CPP 9.20: Poster

Monday, February 25, 2008, 16:45–19:00, Poster A

Lysozyme Adsorption at the Silica/Water Interface: an in situ High Energy X-ray Reflectivity Study — •Florian Evers, Kaveh Shokuie, Michael Paulus, Christian Sternemann, and Metin Tolan — TU Dortmund, Fakultät Physik/DELTA, Maria-Goeppert-Mayer-Str. 2, 44221 Dortmund, Germany

Lysozyme adsorption at the silica/water interface has been studied using high energy x-ray reflectivity which is a well-established method to study the structure of thin films. Data on protein layers adsorbed at solid/liquid interfaces are still rare. The dispersion profile of adsorbed lysozyme layers at hydrophilic silica interfaces, the effect of pH and protein concentration of the aqueous solution on the adsorption, and the time-dependence of the adsorption process were studied. Our observation of lysozyme monolayer adsorption is partially in contrast to former measurements with neutrons and to predictions.