Berlin 2008 – scientific programme
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ST: Fachverband Strahlen- und Medizinphysik
ST 6: Heavy Ion Therapy and Related Basic Research II
ST 6.5: Talk
Tuesday, February 26, 2008, 12:30–12:45, H 2033
Development of new techniques to arrange and recognise cells for radiobiological ion micro beam experiments — •Torsten Koal, Marcus Hohlweg, Tilo Reinhard, and Tilman Butz — Universität Leipzig, Inst. Exp. Phys. II, Linnéstr. 5, 04103 Leipzig, Germany,
Up to a few references the signal ways of the cell-cell-communication (Bystander effects) in micro beam experiments are still unknown. There exist possibilities for both, direct cell-cell-communication via gap junctions as well as a media-provided cell-cell-communication. A promising beginning for the investigation of the communication ways is the compartmentalization of Petri dishes. The compartmentalization allows spatially separated cell-subpopulations to communicate indirectly via medium born factors only. Therefore, Agar, a polysaccharide, was spin coated on Mylar or silicon nitride (Si3N4) irradiation windows. The compartmentalization was realized by proton beam writing on the cell repellent substrate Agar. Then the irradiated areas are soluble in water. It could be shown that cells exclusively grow in the Agar depleted areas.
For future targeted irradiation of defined grown cells, a recognition software named Cellcognition is being developed and will be tested in the next time.