DPG Phi
Verhandlungen
Verhandlungen
DPG

Darmstadt 2008 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe

MO: Fachverband Molekülphysik

MO 1: Biomoleküle

MO 1.5: Vortrag

Montag, 10. März 2008, 15:15–15:30, 3F

Comparison of the fluorescence kinetics of detergent-solubilized and membrane-reconstituted LH2 complexes — •Tobias Pflock1, Manuela Dezi2, Giovanni Venturoli2, Richard J. Cogdell3, Jürgen Köhler1, and Silke Oellerich11Experimentalphysik IV, Universität Bayreuth, D-95447 Bayreuth — 2Dept. of Biology, University of Bologna, Italy — 3GBRC, University of Glasgow, UK

Picosecond time-resolved fluorescence spectroscopy has been used in order to compare the fluorescence kinetics of detergent-solubilized and membrane-reconstituted light-harvesting 2 (LH2) complexes from the bacteria Rhodopseudomonas (Rps.) acidophila and Rhodobacter (Rb.) sphaeroides. LH2 complexes were reconstituted in phospholipid model membranes at different lipid:protein-ratios and all samples were studied exciting with a wide range of excitation densities. While the detergent-solubilized LH2 complexes from Rps. acidophila showed monoexponential decay kinetics (τf = 980 ps) for excitation densities of up to 3· 1013 photons/(pulse· cm2), the membrane-reconstituted LH2 complexes showed multiexponential kinetics even at low excitation densities and high lipid:protein-ratios. The latter finding indicates an efficient clustering of LH2 complexes in the phospholipid membranes. Similar results were obtained for the LH2 complexes from Rb. sphaeroides.

100% | Mobil-Ansicht | English Version | Kontakt/Impressum/Datenschutz
DPG-Physik > DPG-Verhandlungen > 2008 > Darmstadt