Darmstadt 2008 – wissenschaftliches Programm
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MO: Fachverband Molekülphysik
MO 1: Biomoleküle
MO 1.7: Vortrag
Montag, 10. März 2008, 15:45–16:00, 3F
Raman Spectroscopy for the Characterization of Animal Tissue — Toward Molecular Monitoring of Tumorigenicity and Hormonal Changes — •Patrice Donfack1, Jing Shen1,2, Alexander Lerchl1, and Arnulf Materny1 — 1Jacobs University Bremen, Germany — 2Wuhan University, China
Current investigation methods of biological systems are mostly based on time-demanding immunoassays and histopathological examination of sample biopsies. The availability of rapid in-situ techniques would be of great advantage. A potential candidate is Raman spectroscopy (RS), which provides specific molecular fingerprints in a non-invasive and non-destructive way. In our contribution we present two examples for an application of RS for the investigation of animal tissue. (i) Hamsters are strongly influenced by seasonal changes of day and night ratio. Experiments on metabolic or hormonal changes in hamsters unequally exposed to daylight have resulted in changes in the Raman spectra of spleen and liver tissue. (ii) RS was also performed to characterize biomolecular alterations in cells of AKR mice predisposed to leukemia. The Raman spectra of AKR mice spleen tissues show a prominent contribution of the β-sheet conformation, which can be unambiguously assigned to the protein’s amide I band. The intensity ratios of this amide I band and the CH2-deformation line at 1450 cm−1 was found to be different for cancerous and healthy mice. Clear changes could also be observed for the nucleic acid band at approx. 1085 cm−1 and e.g. for a double band at 1606 and 1635 cm−1.