Darmstadt 2008 – scientific programme

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MO: Fachverband Molekülphysik

MO 16: Biomoleküle

MO 16.11: Poster

Tuesday, March 11, 2008, 14:00–19:00, Poster C1

Fluorescence correlation spectroscopy on flavoproteins — •Christian Brock¹, Florian Spreitler², Astrid Pelzmann³, Ortwin Meyer⁴, and Jürgen Köhler⁵ — ¹Lehrstuhl für Experimentalphysik 4, Universität Bayreuth — ²Lehrstuhl für Experimentalphysik 4, Universität Bayreuth — ³Lehrstuhl für Mikrobiologie, Universität Bayreuth — ⁴Lehrstuhl für Mikrobiologie, Universität Bayreuth — ⁵Lehrstuhl für Experimentalphysik 4, Universität Bayreuth

We use fluorescence correlation spectroscopy to investigate the binding properties of CO-dehydrogenase, a flavoenzyme that plays an important role in the respiratory chain of the CO-oxidizing bacterium Oligotropha carboxidovorans. CO-dehydrogenase is labeled with fluorescein, and the change of its diffusion-coefficient upon binding to a lipid vesicle is monitored. This experiment will be repeated with modified versions of the enzyme, lacking certain functional groups that are supposed to be responsible for binding. Furthermore, triplet kinetics of FAD and riboflavin have been analyzed.