Darmstadt 2008 – wissenschaftliches Programm

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MO: Fachverband Molekülphysik

MO 16: Biomoleküle

MO 16.1: Poster

Dienstag, 11. März 2008, 14:00–19:00, Poster C1

Raman Spectroscopy Discrimination of HaCaT and A5RT3 Human Skin Model Cell Lines — •Patrice Donfack, Maren Rehders, Klaudia Brix, and Arnulf Materny — Jacobs University Bremen, Germany

HaCaT and its tumorigenic counterpart A5RT3 cell lines represent good models for studying human skin keratinocytes and carcinoma derived from them. Traditional detection methods of tumor cells relying on immunoblotting are time-consuming. Optical methods are potentially much faster. Fluorescence imaging focuses on the characterization of already known differentiation or dedifferentiation markers. However, Raman spectroscopy (RS) is capable of yielding fingerprint-like characterization of the sample without the necessity of restricting the analysis to few molecules of interest. In our contribution we show the application of RS and surface enhanced RS (SERS) to distinguish between normal HaCaT and tumorigenic A5RT3 cells. Striking differences were revealed in the overall Raman intensity as well as the intensity ratio of the protein amide I band at 1657 and the CH₂ deformation band at 1447 cm⁻¹. These changes were even further pronounced in the SERS experiments. Lipids are more effective Raman scatterers than proteins. The relatively stronger enhancement of the CH₂ deformation band points to a significantly different lipid content in HaCaT. Furthermore, A5RT3 cells showed higher nucleus/cytoplasm ratios with prominent DNA vibrations and a higher but partly altered protein contribution indicating modifications of cellular metabolism and differentiation state.