Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe

MO: Fachverband Molekülphysik

MO 18: Femtosekundenspektroskopie

MO 18.9: Poster

Dienstag, 11. März 2008, 14:00–19:00, Poster C1

Detection of C-Deuterium compounds by CARS microscopy — •Gero Bergner^1,3, Denis Akimov¹, Michael Schmitt^1,2, Sebastian Schlücker³, and Jürgen Popp^1,2 — ¹Institut für Photonische Technologien, Albert-Einstein-Straße 9, 07745 Jena — ²Institut für Physikalische Chemie, Lessingstraße 10, 07743 Jena — ³Institut für Physikalische Chemie, Am Hubland, 97074 Würzburg

Coherent anti-Stokes Raman scattering (CARS) microscopy is a powerful imaging technique with chemical specificity. In particular, it provides vibrational contrast without need of external labelling. Here, we present first steps to establish CARS as a new method for drug localization in living cells. Chemical contrast in the images is obtained by probing the C-D stretch vibration of internally labelled (Deuterium-substituted) compounds. For determining the ratio of Raman-resonant signal to non-Raman-resonant background, both C-D and C-H species of the same compound are investigated. First results for systematically determining the detection limit of this methodology are presented.