Dresden 2009 – scientific programme
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BP: Fachverband Biologische Physik
BP 1: Dynamic Processes and Pattern Formation
BP 1.9: Talk
Monday, March 23, 2009, 12:45–13:00, HÜL 186
Spatiotemporal control of the energy metabolism in a thin layer of yeast cells by oxygen gradients — •Christian Warnke1, Thomas Mair2, Mathias Müller1, Hartmut Witte1, Marcus J. B. Hauser2, and Alois Krost1 — 1Otto-von-Guericke-Universität Magdeburg, Inst. Exp. Phys., Abt. Halbleiterepitaxie — 2Otto-von-Guericke-Universität Magdeburg, Inst. Exp. Phys., Abt. Biophysik
The energy metabolism of cells can work both in absence (anaerobic) and in presence (aerobic) of oxygen. Specially, the anaerobic energy metabolism is represented by glycolysis, a pathway that is characterized by oscillatory behavior. Accordingly, spatiotemporal patterns, resulting from reaction-diffusion coupling, can be observed as well. We present an experimental method to produce spatiotemporal gradients of oxygen in planar yeast cell / Au electrode - interfaces that exhibit glycolytic oscillations. This planar interface allowed a stimulation by electrical pulses of energy metabolism by electrolytic reactions between the blank Au-metal and the electrolytic solution creating oxygen concentration close of the electrodes. The local oxygen pulses are aimed to perturb glycolytic oscillations by a short activation of the aerobic energy metabolism. Additionally, the studies were conducted at different temperatures using a Peltier element connected with the electrode-yeast-interface. We investigated the effect of these local perturbations on the temporal and spatiotemporal dynamics of glycolysis in yeast cells.