Dresden 2009 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 11: Novel Methods

BP 11.7: Vortrag

Dienstag, 24. März 2009, 16:00–16:15, ZEU 260

SERS labels for red laser excitation: silica-encapsulated SAM on tunable gold/silver nanoshells — •Magdalena Gellner, Max Schütz, Bernd Küstner, and Sebastian Schlücker — Department of Physics, University of Osnabrück, 49076 Osnabrück

Silica-encapsulated self-assembled monolayers (SAMs) on tunable gold/silver nanoshells for use as surface-enhanced Raman scattering (SERS) labels in bioanalytical and biomedical applications with red laser excitation are presented. [1] This concept combines the spectroscopic advantages due to the maximum surface coverage and uniform molecular orientation of Raman reporter molecules within a SAM together with the high chemical and mechanical stability of a glass shell. The absorption, scattering and extinction spectra of various gold/silver nanoshells were calculated using Mie theory. Quantitative SERS efficiencies based on theoretical scattering intensities are compared with experimental findings. [2] Our improved SERS label design results in ∼ 180 times brighter SERS signals compared with existing approaches based on single gold nanospheres.[1] Using SERS-labeled antibodies, the selective localization of prostate-specific antigen (PSA) in the epithelium of prostate tissue specimens by immuno-SERS microscopy with red laser excitation is demonstrated.

[1] B. Küstner, M. Gellner, M. Schütz, F. Schöppler, A. Marx, P. Ströbel, P. Adam, C. Schmuck, S. Schlücker, Angew. Chem. Int. Ed., accepted

[2] M. Gellner, B. Küstner, S. Schlücker, Vib. Spectrosc., 2008, doi:10.1016/j.vibspec.2008.07.011