Dresden 2009 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 12: Single Molecules

BP 12.6: Vortrag

Mittwoch, 25. März 2009, 11:15–11:30, HÜL 186

Time resolved three-dimensional orientation of eGFP — •Richard Börner — Universität zu Lübeck, Institut für Physik, Ratzeburger Allee 160, 23568 Lübeck, Germany

Confocal microscopy is a powerful method for single molecule investigation of fluorescent macromolecules. Beside the translatory movement of labeled or autofluorescent molecules rotational dynamics reflect the properties of the macromolecule and its surrounding. In principal the determination of the molecular orientation bases on the defined orientation of the absorption/emission dipole with respect to the molecular frame. Using a method which has been recently proposed by Hohlbein & Hübner [1,2] we demonstrate the time resolved three-dimensional orientation determination for the well known and biological relevant molecule eGFP. By using adapted FCS and TCSPC measurments we observe the orientation on a millisecond down to a nanosecond time scale.

[1] J. Hohlbein & C. G. Hübner, APL, 86, 121104 (2005)

[2] J. Hohlbein & C. G. Hübner, JCP, 129, 094703 (2008)