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BP: Fachverband Biologische Physik

BP 13: Cell Migration

BP 13.2: Vortrag

Mittwoch, 25. März 2009, 11:00–11:15, ZEU 260

Force generation in moving fish keratocytes — •Claudia Brunner, Michael Gögler, Daniel Koch, Thomas Fuhs, Allen Ehrlicher, and Josef Käs — Universität Leipzig

A fundamental step in cell migration is the advancement of the cell's leading edge which is hypothesized to be mediated by actin polymerization against the plasma membrane. Our newly established SFM-technique revealed that the force generating mechanism driving this process is indeed actin polymerization. Cells treated with the actin polymerization inhibitor cytochalasin D generated significantly lower forces. Additionally, we directly measured a force associated with the retrograde flow within the lamellipodium, which demonstrates that the protrusion forces are decoupled from the cell body and are generated exclusively at the leading edge. We show that actomyosin interaction is primarily responsible for cell body and traction force generation while myosin II contraction cannot be the dominant force generating mechanism driving retrograde flow in the central lamellipodium.