Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster II
BP 17.11: Poster
Mittwoch, 25. März 2009, 17:15–19:45, P3
Cell Adhesion and Cell Detachment Forces on Micro-Nanopatterned Substrates — •Janosch Deeg1,2, Ilia Louban1,2, Daniel Aydin1,2, and Joachim Spatz1,2 — 1University of Heidelberg, Dept. of Biophysical Chemistry, Im Neuenheimer Feld 253, D-69120 Heidelberg — 2Max-Planck-Institute for Metals Research, Dept. of New Materials & Biosystems, Heisenbergstr. 3, D-70569 Stuttgart
Au-nanopatterned substrates, produced by micellar block copolymer nanolithography, are used to make adhesion ligands of a cell be positioned like the quasi-hexagonal ordered Au-nanoparticles on the surface. By tuning the spacing of these biofunctionalized nanoparticles, one is able to control the distance between adjacent binding sites. Former experiments have shown that an interparticle distance of more than 73 nm strongly reduces cell spreading, cell detachment forces and the formation of adhesion clusters. Microstructuring of these patterns divides the surface into regions with and without Au-particles due to vary the global density, meaning in this case binding sites per area, not only by changing the distance between these sites, but by creating entire micrometer sized parts without particles next to nanostructured ones. This diploma thesis is mainly interested in how far the detachment force of adherent cells depends on the amount of available integrin binding sites per area in comparison to their distance. The cell detachment force is measured with an AFM by immobilizing the cell on the functionalized tipless cantilever and subsequently detaching it from the surface. We expect to gain a deeper understanding about the effect of integrin spacing and density on cell adhesion strength.