Dresden 2009 – scientific programme
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BP: Fachverband Biologische Physik
BP 17: Poster II
BP 17.14: Poster
Wednesday, March 25, 2009, 17:15–19:45, P3
Protein diffusion in crowded and charged solutions: A light scattering and neutron spin echo study — •Marcus Hennig1,2, Felix Roosen-Runge1,2, Fajun Zhang1, Stefan Zorn1, Maximilian Skoda3, Robert M. J. Jacobs4, Peter Fouquet2, Tilo Seydel2, and Frank Schreiber1 — 1Institut für Angewandte Physik, Universität Tübingen, Germany — 2Institut Laue-Langevin, France — 3ISIS, Didcot, UK — 4Chemistry Research Laboratory, Oxford, UK
Globular proteins under physiological conditions occur in crowded solutions with a protein volume fraction attaining up to approximately 40%. A recent finding of reentrant condensation of proteins induced by polyvalent salts underlines the necessity to examine salt-induced charge effects in order to understand the biological function and dynamical behavior of solvated proteins.
We review different existing models to interpret protein diffusion data in "crowded" solutions and we also consider the effect of charges. We discuss these models in the context of a combined light scattering and neutron spin-echo study of the short-range and long-range nanosecond diffusion of the model globular protein bovine serum albumin (BSA) in aqueous solution as a function of the NaCl salt concentration. The interpretation of the data on the BSA model system is put in the context of existing studies on related systems and of the relevance of charges for protein diffusion and protein function under physiological conditions.