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BP: Fachverband Biologische Physik

BP 17: Poster II

BP 17.28: Poster

Mittwoch, 25. März 2009, 17:15–19:45, P3

Novel PSs for PDT: time-resolved detection of ¹O₂-phosphorescence allows to determine the PS’s localisation — •Christoph Feest¹, Annegret Preuss¹, Beate Röder¹, and Ursula Simonis² — ¹Institut für Physik, Humboldt-Universität zu Berlin — ²Department of Chemistry and Biochemistry, San Fransisco State University

Novel photosensitisers (PS) for Photodynamic Therapy (PDT) have been designed to specifically localize at mitochondria as they play a key role in programmed cell-death (apoptosis). The PSs are based on a Tetraphenylporphyrin core and have specific substitutions that modulate their physico-chemical properties and allow for specific intracellular localisation. The photophysical parameters of all compounds were determined in solution. Additionally, the PDT relevant singlet oxygen (¹O₂) generation was investigated in vitro using flash-photolysis and time resolved ¹O₂ luminescence detection. A new setup was successfully used for evaluation of ¹O₂ consumption during low-dose irradiation of cells. The intracellular localization was investigated in vitro using CLSM and FLIM technique. In the future, the combination of these optical methods for investigation of light-induced photosensitized processes may enable us to precisely determine the intracellular site of the photodynamic action.