Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster II
BP 17.32: Poster
Mittwoch, 25. März 2009, 17:15–19:45, P3
Controlling cell signalling with magnetic nanoparticles — •Verena Schittler1,2, Delphine Arcizet1,2, Yoshihiko Katayama3,2, Don Lamb3,2, Stefan Zahler4, Joachim Rädler1,2, and Doris Heinrich1,2 — 1Department für Physik, Ludwig-Maximilians-Universität, Munich — 2Center of NanoScience (CeNS) — 3Department Chemie und Biochemie, Ludwig-Maximilians-Universität, Munich — 4Department Pharmazie, Ludwig-Maximilians-Universität, Munich
In recent years, numerous biomedical applications for superparamagnetic iron oxide nanoparticles have emerged as targeted drug delivery and magnetic resonance imaging. Labelling these nanoparticles by lipophilic dyes to visualize the nanoparticles via fluorescence microscopy offers new potential for imaging.
Our research is focused on cell control by fluorescent magnetic nanoparticles in living cells and we study the impact of external magnetic forces on transport properties inside the cell and on cell migration as a whole. So far, we investigated the internalisation in Dictyostelium discoideum cells and in human mammary epithelial cells (HMEC) with and without force field. The fluorescence of the particles allows us to visualize this step. To analyse further the intracellular diffusion and active transport by molecular motors of the particles, we use a 3D-tracking setup which offers the possibility to follow the particles online also in the z-direction. We aim at a better understanding of cell migration by stimulating magnetically labelled cells with external magnetic forces and investigate exact mechanisms in magnetotactic response.