Dresden 2009 – scientific programme
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BP: Fachverband Biologische Physik
BP 19: Cell Adhesion
BP 19.9: Talk
Thursday, March 26, 2009, 13:00–13:15, ZEU 260
Artificial three-dimensional scaffolds for cell adhesion studies — •Thomas Striebel1, Franziska Klein2, Martin Wegener3, Martin Bastmeyer2, and Ulrich S. Schwarz1 — 1University of Karlsruhe, Theoretical Biophysics Group, Kaiserstrasse 12, 76131 Karlsruhe, Germany — 2University of Karlsruhe, Institute of Zoology, Haid-und-Neu-Strasse 9, 76131 Karlsruhe, Germany — 3University of Karlsruhe, Institute of Applied Physics, Wolfgang-Gaede-Str. 1, 76131 Karlsruhe, Germany
Adhesion of tissue cells is traditionally studied on two-dimensional culture dishes. In this way, much has been learned how environmental stimuli determine the cellular response, including migration, proliferation and fate. However, much less is known about how tissue cells behave in three-dimensional environments. We have used direct laser writing to design three-dimensional scaffolds for cell adhesion studies with feature sizes down to 100 nm. Our setup can be used to produce structures with many different geometries in a short time and gives highly reproducible results. By applying our procedure to different photoactive materials, we were able to vary the stiffness of the scaffolds and to optimize the system for imaging. Using quantitative image processing, we now can analyze shape, traction and adhesion structures of cells in three dimensions.