Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Poster I
BP 7.16: Poster
Montag, 23. März 2009, 17:45–20:00, P3
In vitro assembly and characterization of keratin 8/18 intermediate filaments — •Anke Leitner1, Norbert Mücke3, Tatjana Wedig2, Harald Herrmann2, Michael Beil4, and Othmar Marti1 — 1Department of Experimental Physics, Ulm University, Ulm, Germany — 2Division of Molecular Genetics, German Cancer Research Center, Heidelberg, Germany — 3Division Biophysics of Macromolecules, German Cancer Research Center, Heidelberg, Germany — 4Department of Internal Medicine I, Ulm University, Ulm, Germany
In order to find out more about the mechanical properties of the keratin cytoskeleton it is usefull to have a look on in vitro assembled keratin filaments. In the work presented here we will show nanomechanical properties of the assembled keratin 8/18 filaments. The investigations are done by means of atomic force microscope (AFM). Two different substrates and ways of sample preparation are used. From the 2-dimensional AFM images we calculate the persistence length of the keratin 8/18 filaments with the worm-like-chain model.