Dresden 2009 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 7: Poster I

BP 7.36: Poster

Montag, 23. März 2009, 17:45–20:00, P3

Monitoring single membrane proteins in an anti-Brownian electrokinetic (ABEL) trap — •Anastasiya Golovina-Leiker, Nawid Zarrabi, Monika Düser, Rolf Reuter, Jörg Wrachtrup, and Michael Börsch — 3.Physikalisches Institut, Universität Stuttgart, Germany

Brownian motion prevents the observation of one biomolecule for extended periods. Adam E. Cohen and W. E. Moerner have developed an anti-Brownian electrokinetic (ABEL) trap to trap individual protein molecules in free solution, under ambient conditions, without requiring any attachment to beads or surfaces [Cohen and Moerner, PNAS 2006). They also demonstrate trapping and manipulation of single lipid vesicles. We present an extension of their approach to trap membrane proteins reconstituted in lipid vesicles and to simultaneously monitor the conformational changes of the active enzyme by fluorescence resonance energy transfer.