Dresden 2009 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 28: POSTERS Interfaces and Thin Films
CPP 28.2: Poster
Mittwoch, 25. März 2009, 17:00–19:00, P3
Supported Lipid Bilayers on Spacious and pH Responsive Polymer Cushions with Varied Hydrophilicity — •Lars Renner1, Tilo Pompe1, Regis Lemaitre2, David Drechsel2, and Carsten Werner1 — 1Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Germany — 2Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
We report on the successful formation of supported multicomponent lipid bilayer membranes (sLBMs) from natural occurring lipids as well as synthetic lipids on a set of polymer cushions consisting of alternating maleic acid copolymers. Maleic acid copolymers provide a versatile platform to adjust the physico-chemical behaviour by the choice of the comonomer unit. The formation of sLBMs was triggered by a transient reduction of the electrostatic repulsion between the polymer cushions and the lipid vesicles by lowering the solutions pH to 4. Upon formation the stability of sLBMs was not affected by subsequent variations of the environmental pH to 7.2. The degree of hydrophilicity and swelling of the anionic polymer cushions was found to determine both the kinetics of the membrane formation and the mobility of the sLBM. An increase in cushion hydrophilicity correlated with a strong increase in the diffusion coefficient of the lipids. The observations strongly support the important role of the support's polarity for the fluidity of the sLBM. The investigated polymer cushions are considered to open new options for the in situ modulation of sLBM characteristics for the successful integration of functional transmembrane proteins.