Dresden 2009 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 34: Microfluidics I: Applications and Devices

CPP 34.4: Vortrag

Donnerstag, 26. März 2009, 10:15–10:30, ZEU 160

Static and dynamic surface-coatings in quartz crystal- and Poly(dimethylsiloxane)-microfluidic channels — •Martina Everwand, Jan Regtmeier, and Dario Anselmetti — Bielefeld University, Experimental Biophysics & Applied Nanoscience, Universitätsstr. 25, 33615 Bielefeld, Germany

For bioanalytical applications, the surface properties of the Lab-on-a-Chip device are of great relevance, e.g. for limiting the band broadening in electroosmotic flow applications.

Therefore we have investigated three different coatings Poly(L-lysine)-poly(ethylene glycol) (PLL-PEG), F108 and n-Dodecyl-D-Maltoside Methyl Cellulose (DDM-MC)) in quartz-crystal and Poly(dimethylsiloxane) (PDMS) microfluidic systems with respect of their electroosmotic flow, protein adhesion and contact angle properties. We used either statical or dynamical coating, where the polymers were incubated and rinsed prior usage or where the polymers were directly added to the separation solution.

In summary, all three coatings were able to reduce the electroosmotic flow and protein adsorption in quartz as well as in Poly(dimethylsiloxane) microchips. The highest reductions were obtained for the dynamically coated n-Dodecyle-Maltoside Cellulose polymer.