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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 37: Biopolymers (joint session CPP/BP)

CPP 37.7: Vortrag

Donnerstag, 26. März 2009, 16:15–16:30, ZEU 114

Proteins on Surfaces investigated by Microbeam Grazing Incidence Small Angle X-ray Scattering — •Ronald Gebhardt¹, Christian Riekel¹, Charlotte Vendrely², Manfred Burghammer¹, and Peter Müller-Buschbaum³ — ¹European Synchrotron Radiation Facility, B.P. 220, F-38043 Grenoble Cedex, France — ²Université de Cergy-Pontoise, ERRMECE, F-95000, Cergy-Pontoise, France — ³TU München, Physik Department E13, München, Germany

Grazing incidence small angle scattering with a 1 micron x-ray beam (μGISAXS) is applied to study structural ordering of casein micelles and fibroin in solution cast films. μGISAXS scans provide the possibility to locate highly ordered areas and to investigate variation in the molecular packing.

In the case of the casein micelles, ordered film structures have been generated by decreasing their natural size dispersion. While dynamic light scattering was used to characterize the different size fractions in solution, μGISAXS and roughness are measured on the resulting casein films. GISAXS-Patterns are analyzed by simulations providing the dimension and nearest neighbor distances of casein micelles.

In the case of fibroin, ordering of nano-fibers formed during the drying process is investigated. The experimental data are analyzed by simulations and compared to SEM, AFM and Raman scattering experiments.