Hamburg 2009 – scientific programme
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MO: Fachverband Molekülphysik
MO 13: Molekülphysik Poster 1
MO 13.28: Poster
Tuesday, March 3, 2009, 16:30–19:00, VMP 8 Foyer
Raman Study of AKR/J Mice Spleen at Different Excitation and Bleaching Profiles, and of Hormonal and Tumorigenic Changes in Hamsters — •Patrice Donfack, Alexander Lerchl, and Arnulf Materny — Jacobs University Bremen, Germany
We present Raman spectroscopic studies of transgenic AKR/J mouse spleen tissue under green and red excitation, and of hamster liver, thymus, and testes tissue under red excitation, in combination with multivariate analysis. With green excitation, healthy and cancerous spleen showed slight changes in the intensity ratio I1672/I1450, different amide III bands, reduced intensity of the phenylalanine (Phe) band, and especially stronger nucleic acid (e.g. DNA: 1085 cm−1) bands in tumors and a double fine structure at 1606 and 1639 cm−1 in different aspect ratios. Under red excitation, the intensity of the Phe band drops notably in tumors and could be used for both differentiation and grading. Using different excitations, we show that some characteristic features could only be resolved for certain wavelengths. Moreover, the fluorescence bleaching time profile under green excitation provided insight into the actual bleaching effect and the retained aspect ratio of the double fine structure at 1606 and 1639 cm−1 is consistently correlated with tumorigenicity. Hamsters subjected to seasonal changes of day/night ratios experience fur color change, and can show enlarged testes in short day periods. With Raman spectra of liver, thymus, and especially of testes tissue, matched with short and long day hamsters, we hypothesize on eventual hormonal changes, and a Raman indication of tumorigenesis in short day hamsters with enlarged testes.