Hamburg 2009 – wissenschaftliches Programm

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MO: Fachverband Molekülphysik

MO 18: Experimentelle Techniken

MO 18.1: Hauptvortrag

Donnerstag, 5. März 2009, 10:30–11:00, VMP 6 HS-F

Disturbing spectral interferences and their suppression in Femtosecond Stimulated Raman Microscopy (FSRM) — Benjamin Marx, Evelyn Ploetz, and •Peter Gilch — Lehrstuhl für Biomolekulare Optik und CIPSM, Department für Physik, Ludwig-Maximilians-Universität, Oettingenstr. 67, 80538 München, Germany

Femtosecond Stimulated Raman Microscopy (FSRM) is an upcoming technique in non-linear microscopy with the capability to image chemical structures non-invasively [1]. It employs femtosecond white light pulses as probe pulses and intense picosecond pulses as pump pulses. At focus of the scanning microscope stimulated Raman scattering occurs. Chemical entities in the sample are identified via the spectral signature of this process. A disturbing effect is the broadening of the pump pulse due to self phase modulation in the focal region of the microscope leading to spectral interference on the detector. This interference reduces the signal to noise ratio. Slightly modulating the optical path difference between pump and probe by a piezo stage suppresses this effect.

[1] E. Ploetz, S. Laimgruber, S. Berner, W. Zinth, P.Gilch, Appl. Phys. B 87, 389-393 (2007)