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MO: Fachverband Molekülphysik
MO 2: Biomoleküle 1
MO 2.5: Vortrag
Montag, 2. März 2009, 15:00–15:15, VMP 6 HS-F
Light-harvesting complexes in a membrane environment: A time-resolved fluorescence study — •Tobias Pflock1, Florian Spreitler1, June Southall2, Richard Cogdell2, Jürgen Köhler1, and Silke Oellerich1 — 1Experimentalphysik IV, Universität Bayreuth, D-95447 Bayreuth — 2Glasgow Biomedical Research Centre, University of Glasgow, UK
Picosecond time-resolved fluorescence spectroscopy has been used to investigate the intercomplex energy-transfer properties of membrane-reconstituted light-harvesting 2 (LH2) complexes from the purple bacteria Rhodopseudomonas acidophila. LH2 complexes were reconstituted in phospholipid model membranes at different lipid-protein ratios and all samples were excited with a wide range of excitation densities and excitation rates.
In order to explain the fluorescence decay data, we use a nonlinear decay model. This model takes annihilation of excitation into account, which is interpreted as a signature of self-organized clustering of the LH2 complexes within the lipid bilayer environment. This conclusion is corroborated by AFM data.