Hannover 2010 – wissenschaftliches Programm

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MO: Fachverband Molekülphysik

MO 26: Poster: Biomolecules

MO 26.2: Poster

Donnerstag, 11. März 2010, 16:00–18:30, Lichthof

Probing Molecular Profiles of Tumorigenicity in Colon Carcinoma Differential Tumor Cells Associated with a Tumor-Specific Lipid Marker by Raman Spectroscopy — •Patrice Donfack¹, Gabriele Multhoff², and Arnulf Materny¹ — ¹Jacobs University Bremen, Germany — ²Klinikum rechts der Isar, TU München, Germany

Studying detailed differentiation steps of tumorigenicity is a rather challenging task, which leads towards the understanding of the molecular details of tumorigenicity. We present Raman spectroscopic studies of differential tumorigenic CX- and CX+ cells, in combination with multivariate analysis. These cells are sublines of human colon carcinoma cell lines, with differential Hsp70 membrane expression pattern associated with a tumor-specific lipid component. Raman spectroscopy has revealed protein-dominated spectra of CX- and CX+ with slight differences in the lower wavenumber region. However, changes in the protein bands around 2933 cm⁻¹, where prominent lipid bands are expected, are more dramatic. These observations support the assumption that tumorspecific lipid markers interact with key proteins that play an important role in tumor progression such as cell growth regulation. Changes in the cell’s lipid composition lead to changes in important protein components. Changes observed for CX- and CX+ have been subjected to multivariate analysis for resolving the Raman signatures of tumorigenicity from the bands at 1094, 1300, 1450, 1656, and about 2933 cm⁻¹. Further insights can be gained by surface enhanced Raman scattering of the CX-/CX+ pecific lipid globoyltriaosylceramide Gb3.