Hannover 2010 – scientific programme

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MO: Fachverband Molekülphysik

MO 32: Experimental Techniques II

MO 32.2: Talk

Friday, March 12, 2010, 14:15–14:30, F 142

Vibrational imaging based on stimulated Raman scattering microscopy — •G. Hehl, P. Nandakumar, A. Kovalev, S. Gomes da Costa, and A. Volkmer — 3rd Institute of Physics, University of Stuttgart, Stuttgart, Germany

Based on stimulated Raman gain and loss detection, we demonstrate noninvasive point-by-point vibrational mapping of chemical and biological samples with high-sensitivity and without the requirement for labeling of the sample with natural or artificial fluorophores. A stimulated Raman scattering (SRS) microscope with near-infrared picosecond laser pulses at high repetition rates and radio-frequency lock-in detection is accomplished [1]. We experimentally demonstrate a major benefit of this technique, which is the capability to respond exclusively to the linear Raman-resonance properties of the sample, thus allowing a direct quantitative interpretation of image contrast in terms of the number density of Raman-active modes.

[1] P. Nandakumar, A. Kovalev, and A. Volkmer, New J. Phys. 11 (2009) 033026.