Hannover 2010 – scientific programme
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MO: Fachverband Molekülphysik
MO 5: Femtosecond Spectroscopy I
MO 5.6: Talk
Tuesday, March 9, 2010, 15:15–15:30, F 102
Isotope labeled riboflavin studied by sub-ps time-resolved infrared spectroscopy — •Matthias M. N. Wolf1, Tatiana Domratcheva2, Katharina Chevalier1, and Rolf Diller1 — 1Fachbereich Physik, TU Kaiserslautern, D-67663 Kaiserslautern, Germany — 2Max-Planck-Institut für medizinische Forschung, D-69120 Heidelberg, Germany
Biological blue light receptors as Cryptochromes, AppA or Phototropins utilize the characteristic blue light absorption of Flavin chromophores. In our previous work [1] we investigated excited state vibrational modes and dynamics of Riboflavin in DMSO with sub-picosecond time resolved infrared spectroscopy. Assignment of electronic ground and first excited state vibrational modes was performed via quantum chemical calculations.
Here we present measurements and calculations of two isotope labeled Riboflavin compounds. The results clarify the assignment of carbonyl vibrations and their involvement in various hydrogen-bonding schemes that might be relevant for native protein systems. In addition variation of excitation wavelength and its effect on picosecond dynamics will be discussed.
[1] M. M. N. Wolf et. al., J. Phys. Chem. B 112 13424-13432 (2008).