Hannover 2010 – wissenschaftliches Programm

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Q: Fachverband Quantenoptik und Photonik

Q 41: Ultrashort Laser Pulses: Applications III

Q 41.8: Vortrag

Mittwoch, 10. März 2010, 18:15–18:30, F 342

Ground Sted Depletion (GSD) Nanoscopy of NV Color Centers in Diamond with Single Digit Resolution — •Dominik Wildanger, Eva Rittweger, and Stefan W. Hell — Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany

We present a new implementation of fluorescence microscopy based on the depletion of the fluorophores ground state providing a resolution far beyond the diffraction limit. Therefore we utilize an intense excitation beam which features an intensity minimum at its center (e.g. a doughnut shaped beam). Given high enough laser power the ground state of fluorophores in close vicinity to the minimum is depleted while a fluophore at the very center remains in the ground state. Scanning through the sample leads to a negative high resolution image. In order to obtain a positive image the picture has to be deconvolved with the excitation PSF. As an alternative to the negative images we show a pump probe configuration in which we probe the color centers that remain in the ground state with a second, chopped excitation beam. Subtraction of both signals with a lock-in amplifier renders directly a positive image. A lateral resolution of approx. 8 nm was obtained. Our study underscores the key role of exploiting (molecular) states for overcoming the diffraction barrier in far-field optical microscopy