Hannover 2010 – wissenschaftliches Programm
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Q: Fachverband Quantenoptik und Photonik
Q 55: Poster II
Q 55.80: Poster
Donnerstag, 11. März 2010, 16:00–19:00, Lichthof
Chlorophyll fluorescence measurements with single cells of the photosynthetic model organism Chlamydomonas reinhardtii — •Andreas Garz1, Stefanie Schlede2, Heiko Lokstein2, and Ralf Menzel1 — 1Institut für Physik und Astronomie/Photonik — 2Institut für Biochemie und Biologie/Pflanzenphysiologie, Universität Potsdam, Karl-Liebknecht-Str. 24/25, 14476 Potsdam
A comprehensive systems biology analysis of photosynthesis and its regulation in response to selected environmental factors and at different growth stages in a model algal system, Chlamydomonas reinhardtii, and the integration of the obtained insight with research on model higher plants and crop plant species are the aims of the interdisciplinary collaborative Potsdam-Golm (Germany) network GoFORSYS.
Our particular field of interests in this regard is the study of the regulation of photosynthesis in response to environmental factors and at different developmental stages, also at the level of single cells using the principle of puls-amplitude-modulated fluorometry to measure photosynthetic parameters such as photochemical and non-photochemical quenching. The data will be used to gain deeper insight into photosynthesis, its regulation and photoprotective mechanisms by application of systemic modelling.
The employed setup is based on an inverse confocal microscope. For manipulation of single cells during the measurements a home-made optical trap is used. Furthermore, the cells can be manipulated in specifically designed micofluidic assemblies (in the range of twenty microns).