Hannover 2010 – scientific programme
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Q: Fachverband Quantenoptik und Photonik
Q 55: Poster II
Q 55.82: Poster
Thursday, March 11, 2010, 16:00–19:00, Lichthof
Realization of a scanning microscope for nonlinear microscopy — •Sebastian Beer1,2, Petra Groß1, Carsten Cleff1, Lisa Kleinschmidt1, and Carsten Fallnich1 — 1Institut für Angewandte Physik, Westfälische Wilhelms-Universität, Corrensstr. 2, 48149 Münster — 2Fachhochschule Gießen-Friedberg, Wiesenstr. 14, 35390 Gießen
During the last decade, research in biology and the life sciences has inspired and led to major achievements in nonlinear microscopy. This includes the improvement of resolution beyond the diffraction limit as well as advances in and application of different nonlinear microscopy methods like two-photon excited fluorescence, harmonic generation or coherent anti-Stokes Raman scattering (CARS). Typically, these methods require scanning of the focus across the sample.
We have developed a multimodal scanning microscope, where not the focus, but the sample is scanned in order to disturb the focus as little as possible. The easy access to all building blocks and the resulting flexibility made an experimental setup more attractive than a commercial, but expensive microscope. Using a piezo-driven three axis stage, the specimen can be scanned by 150 µm in each direction. We present practical considerations and technical details which one has to keep in mind when constructing a high performance scanning microscope. We also show first results obtained by two-photon excited fluorescence-, second harmonic generation-, and CARS-microscopy, which demonstrate that our microscope is highly suitable for multimodal nonlinear microscopy.