Hannover 2010 – wissenschaftliches Programm

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SYDI: Symposium Diffractive Imaging

SYDI 1: Imaging of biological systems

SYDI 1.4: Hauptvortrag

Freitag, 12. März 2010, 12:00–12:30, E 415

Coherent Diffractive Imaging at LCLS — •Henry Chapman — CFEL, DESY, Hamburg, Germany

The ultrafast pulses from X-ray free-electron lasers may enable the determination of structures of proteins that cannot be crystallized. The specimen would be completely destroyed by the pulse, but that destruction will ideally only happen after the termination of the pulse. In order to address the many challenges that we face in attempting molecular diffraction, we have carried out experiments in coherent diffraction from protein nanocrystals at the Linac Coherent Light Source (LCLS) at SLAC. The periodicity of these objects gives us much higher scattering signals in order to determine the effects of pulse duration and fluence on the high-resolution structure of single objects. The crystals are filtered to sizes less than 2 micron, and delivered to the pulsed X-ray beam in a liquid jet. Diffraction patterns are recorded at the LCLS repetition rate with pnCCD detectors. Preliminary results will be presented on our first LCLS experiments.

This work was carried out as part of a collaboration, for which Henry Chapman is the spokesperson. The collaboration consists of CFEL DESY, Arizona State University, SLAC, Uppsala University, LLNL, The University of Melbourne, LBNL, the Max Planck Institute for Medical Research, and the Max Planck Advanced Study Group (ASG) at the CFEL. The experiments were carried out using the CAMP apparatus, which was designed and built by the Max Planck ASG at CFEL. The LCLS is operated by Stanford University on behalf of the U.S. Department of Energy, Office of Basic Energy Sciences.