Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 13: Nanoparticles and Viruses
BP 13.3: Vortrag
Dienstag, 23. März 2010, 14:45–15:00, H45
Electron microscopic analysis of particle uptake by lung macrophages in a murine allergic asthma model — •Christoph Wigge1, Melanie Conrad2, Holger Garn2, Harald Renz2, and Marianne Geiser1 — 1Institute of Anatomy, University of Bern, Switzerland — 2Department of Clinical Chemistry and Molecular Diagnostics, Philipps University of Marburg, Germany
Efficient particle uptake by lung surface macrophages is essential for the clearance of particles deposited in the peripheral lungs. Thereby, uptake of nanoparticles is of special interest, since there is evidence from epidemiology for a toxicological role of such particles. We investigated particle uptake by cells obtained from bronchoalveolar lavage of mice with induced allergic inflammation in comparison to cells obtained from healthy animals. Cells cultured on porous filter inserts were exposed to microparticles (3-um fungal spores) and to nanoparticles (20-nm gold) for 2 and 4 hours, respectively, and then processed for conventional transmission electron microscopy and electron tomography. We found phagocytic uptake of microparticles by macrophages in all animals, as the vesicular membrane was tightly apposed to the particles. There was evidence for rather unintentional uptake of nanoparticles, which were found in large vesicles containing other material. Electron tomography allowed detailed spatial resolution of nanoparticles in vesicles. In allergic animals, nanoparticles were also found in eosinophils. Uptake of nanoparticles by other leukocytes may contribute to nanoparticle clearance from the inner surface of lungs in inflammation.