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BP: Fachverband Biologische Physik
BP 13: Nanoparticles and Viruses
BP 13.7: Vortrag
Dienstag, 23. März 2010, 16:00–16:15, H45
Nsp7-Nsp8 Supercomplex Building of Fe-CoV — •Henning Seidel1, Yibei Xiao2, Rajesh Ponnusamy2, Rolf Hilgenfeld2, and Christian G. Hübner1 — 1Institute of Physics, Ratzeburger Allee 160, 23538 Lübeck, Germany — 2Institute of Biochemistry, Ratzeburger Allee 160, 23538 Lübeck, Germany
Coronaviruses are enveloped positive-stranded RNA viruses. They possess the largest known RNA genome. Their RNA-dependent RNA-polymerase (RdRp) activity is supplied by the non-structural protein 12 (nsp12) [1]. For SARS-CoV, it was shown that coronaviruses also encode a second RdRp build from nsp7 and nsp8. This hexadecameric nsp7-nsp8 supercomplex is a hollow, cylinder-like structure assembled from eight copies of nsp8 and held together by eight nsp7 molecules [2]. We are aiming at understanding the assembly process and related conformational changes of the supercomplex for the related Feline Coronavirus. The structural and functional examination of the nsp7-nsp8 supercomplex building should help in understanding the replication and transcription mechanisms of Fe-CoV and other coronaviruses like SARS-CoV. In order to gain knowledge of the complex building, we apply methods of single molecule fluorescence, namely fluorescence correlation spectroscopy (FCS) and fluorescence resonance energy transfer (FRET).
[1] Imbert I. et al., The EMBO Journal, Vol 25, 4933-4942 (2006)
[2] Zhai Y. et al., Nature Structural and Molecular Biology, Vol 12, No 11, 980-986 (2005)