Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Physics of Cells II
BP 15.1: Vortrag
Dienstag, 23. März 2010, 14:30–14:45, H43
Formation of long lived local protein kinase C clusters after short Calcium puffs — •Mike Bonny1, Martin Peglow1, Lars Kaestner2, Peter Lipp2, Heiko Rieger1, and Karsten Kruse1 — 1Department of Theoretical Physics, Saarland University, D-66041 Saarbrücken, Germany — 2Institute for Molecular Cell Biology, Medical Faculty of the Saarland University, D-66421 Homburg/Saar, Germany
Conventional protein kinases C (cPKCs) play an important role in signal transduction and in gene regulation. PKCα, a member of the cPKC-family, translocates to
the plasma membrane after activation via cytosolic Ca2+ ions. In particular, there exist local translocation events, when PKCα forms clusters on the membrane
with limited spatial spreads (<4µm). The lifetime of brief events is 400-1500ms, while long lasting events have a lifetime larger than 5s, which markedly exceeds the
duration of a Calcium puff [1].
We show theoretically that allosteric effects together with interactions between membrane-bound PKCα can lead to the observed behaviour.
Using fluorescence resonance energy transfer (FRET) measurements we support our
assumption of so far unknown interactions between PKCα molecules.
Reither, G., Schaefer, M., Lipp, P. (2006). PKCα: a versatile key for decoding the cellular calcium toolkit. JCB 174: 521-533