Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 2: New Technologies
BP 2.9: Vortrag
Montag, 22. März 2010, 12:45–13:00, H43
Programmable Lab on a Chip System for single cell analysis — •Stefan Thalhammer1 and Achim Wixforth2 — 1Helmholtz Zentrum München, Institut für Strahlenschutz, Ingolstädter Landstrasse 1, 85764 Neuherberg — 2Universität Augsburg, Experimental Physiks I, Universitätsstrasse 1, 86159 Augsburg
The collection, selection, amplification and detection of minimum genetic samples became a part of everyday life in medical and biological laboratories, to analyze DNA-fragments of pathogens, patient samples and traces on crime scenes. Here, a multifunctional programmable Lab-on-a-Chip driven by nanofluidics and controlled by surface acoustic waves (SAW) is presented. This system combines serial DNA-isolation-, amplification- and array-detection-process on a modified glass-platform. The fluid actuation is controlled via SAW by interdigital transducers implemented in the chemical modified chip surface. The chemical surface modification allows fluid handling in the sub-microliter range. Minute amount of sample material is extracted by laser-based microdissection out of e.g. histological sections at the single cell level. A few picogram of genetic material are isolated and transferred via a low-pressure transfer system (SPATS) onto the chip. Subsequently the genetic material inside single droplets, which behave like "virtual" beaker, is transported to the reaction and analysis centers on the chip surface via surface acoustic waves, mainly known as noise dumping filters in mobile phones. At these "biological reactors" the genetic material is processed, e.g. amplified via polymerase chain reaction methods, and genetically characterized.