Regensburg 2010 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 25: Focus: Charge Effects in Soft and Biological Matter I (joint CPP, BP, ST)

BP 25.2: Vortrag

Donnerstag, 25. März 2010, 11:30–11:45, H45

Dielectrophoresis: a new tool for continuous DNA/protein interaction studies — •Martina Everwand, Dario Anselmetti, and Jan Regtmeier — Experimental Biophysics & Applied Nanoscience, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld

The investigation of DNA-protein interactions is of central interest in today`s proteomic research like for the metabolic pathway analysis. Here, a novel microfluidic device is presented, which allows efficient separation of protein-complexed DNA from native DNA strands in continuous mode.

The Lab-on-chip device consists of a 3D-structured microfluidic channel network incorporating an integrated barrier with nanometer dimension, that allows for electrodeless dielectrophoresis of DNA-protein complexes.

For the first time, we demonstrate that differently sized DNA fragments as well as DNA/protein and DNA/antibiotics complexes can be continuously separated from unbound DNA at a nano-microfluidic interface.