Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 26: From Single-Molecule to Tissue Dynamics
BP 26.7: Vortrag
Donnerstag, 25. März 2010, 16:00–16:15, H43
Origin and Spatial Distribution of Forces in Motile Cells — Claudia Brunner1, Michael Gögler1, Allen Ehrlicher1, Daniel Koch1, Thomas Fuhs1, Charles Wolgemuth2, and •Josef A. Käs1 — 1Division of Soft Matter Physics, Department of Physics, University of Leipzig — 2Department of Cell Biology and the Center for Cell Analysis and Modeling, University of Connecticut Health Center
Inspired by ambivalent data of individual cellular forces we provide a first complete and consistent set of forces that act in a moving cell measured by a novel SFM technique. Besides contributions from blebbing and hydrodynamics flows it was generally believed that the protrusion of a migrating cell*s leading edge is driven by actin polymerization. Our force measurements modulated by various cytoskeletal drugs show that hydrodynamics flows are negligible and solely actin polymerization drives the advancement of the central lamellipodium. Moreover, we measure the retrograde forces in the midst of the lamellipodium, the central missing link to understand how forces are balanced in motile cells. While the motions in the central lamellipodium, i.e. protrusion and retrograde flow, are solely driven by polymerization and depolymerization forces, the lamellipodial wings and the forces that pull the cell body along rely heavily on contractile actin-myosin interactions. The traction forces in the wings significantly contribute to the local retrograde flow and are the origin of strong forces that advance the cell body.