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BP: Fachverband Biologische Physik
BP 29: Biomolecular Spectroscopy
BP 29.2: Talk
Thursday, March 25, 2010, 14:45–15:00, H45
Multivariate analysis for surface-enhanced Raman scattering (SERS) probe multiplexing and imaging in biological matrices — •Andrea Matschulat1,2, Daniela Drescher1,2, and Janina Kneipp1,2 — 1Institut für Chemie, HU, Brook-Taylor-Str. 2, 12489 Berlin — 2Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Str.11, 12489 Berlin
Raman Spectroscopy as a non-destructive spectroscopic technique allows the study of vibrational fingerprints by which chemical and biological compounds can be identified. An improvement of the spatial resolution on the nm-scale is provided by local optical fields surrounding plasmonic nanostructures which are excited by the incident electromagnetic field. Such so-called surface-enhancement provides more sensitive detection. SERS has therefore attracted considerable interest for its application in bioanalytical chemistry. SERS offers numerous opportunities in the study of spectral changes during molecular interactions in complex biosystems. We demonstrate a multivariate approach for SERS hybrid probe multiplexing and imaging implementing principal component analysis and cluster algorithms. As a first application, we introduced two biocompatible Raman reporter molecules attached to Au nanoaggregates into living 3T3-cells. Such a hybrid probe approach enables the identification of different SERS probes in multiplexed experiments. We present results of hyperspectral mapping analysis providing us information about the cellular uptake, localization and amount of both reporter molecules inside the biosystem.