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BP: Fachverband Biologische Physik

BP 29: Biomolecular Spectroscopy

BP 29.3: Vortrag

Donnerstag, 25. März 2010, 15:00–15:15, H45

Characterization of artificial peptide receptors by UV resonance Raman spectroscopy and non-negative matrix factorization — •Christoph Herrmann¹, Stephan Niebling¹, Sunil Kumar Srivastava¹, Carsten Schmuck², and Sebastian Schlücker¹ — ¹Fachbereich Physik, Universität Osnabrück, Barbarastr. 7, 49069 Osnabrück — ²Institut für Organische Chemie, Universität Duisburg-Essen, Universitätsstr. 5, 45141 Essen

Guanidiniocarbonyl pyrroles are artificial peptide receptors which serve as model systems for investigating the principles of peptide binding. Their carboxylate binding site (CBS) can be selectively monitored by UV resonance Raman (UVRR) spectroscopy. UVRR spectra of guanidiniocarbonyl pyrroles we recorded at different pH values in the range of 4 to 9 in order to characterize the corresponding acid/base equilibrium. By using non-negative matrix factorization (NMF), we were able to extract the pure spectra of the neutral and protonated CBS species from the experimental UVRR spectra. This allowed the quantification of their relative contributions and the site specific pKa determination of the CBS in these artificial peptide receptors.