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BP: Fachverband Biologische Physik

BP 29: Biomolecular Spectroscopy

BP 29.8: Vortrag

Donnerstag, 25. März 2010, 16:30–16:45, H45

Describing transient Fluorescence induction curves by modelling the electron transfer of photosystem II — •Joachim Börner¹, Franz-Josef Schmitt¹, Hans Joachim Eichler¹, Athina Zouni², and Gernot Renger² — ¹Institute of Optics and Atomic Physics, Berlin Institute of Technology, Germany — ²Max-Volmer-Laboratory for biophysical chemistry, Berlin Institute of Technology, Germany

In this work a generalised PS II model for the simulation of fluorescence induction curves is presented. Fluorescence induction curves were measured with continuous laser illumination on isolated core complexes from the thermophilic cyanobacterium Thermosynechococcus elongatus and hole cells of the green algae Chlorella pyrenodoisa chick. Perfect data fit was achieved within the framework of a model for the PS II reaction pattern comprising electron transfer reactions to the exogenous electron acceptor K₃Fe(CN)₆ in core complexes and to the endogenous plastoquinone in chlorella cells. Based on data reported in the literature a consistent set of rate constants was obtained for electron transfer at the donor and acceptor side of PS II. The simulations based on the model of the PS II reaction pattern provide information on the time courses of population probabilities of different PSII states in photosynthetic samples under various conditions (e.g. presence of herbicides, other stress conditions, excitation with actinic pulses of different intensity and duration).

Acknowledgement: Financial support by DFG project SfB 429 and Federal Ministry of Education and Research (BMBF)