Regensburg 2010 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 3: DNA, RNA and Associated Enzymes

BP 3.1: Hauptvortrag

Montag, 22. März 2010, 14:00–14:30, H43

Exciting positional control with DNA Origami: Onwards nanoscale gadgets for Science and Technology. — •Hendrik Dietz — Laboratory for Biomolecular Nanotechnology, Physik Dept, Technische Universität München - Garching, Germany

Scaffolded DNA Origami (1) is a molecular self-assembly method that enables folding a multiple-kilobase 'back-bone' DNA molecule into complex nanoscale shapes by introducing interactions between different segments on the backbone molecule. Interaction patterns are expressed by sets of synthetic 'staple' molecules that are added to the much longer back-bone molecule. Based on this concept we have developed a general approach to the construction of custom three-dimensional shapes that can be conceptualized as creating custom-crossection bundles of DNA double helices (2) where the number, arrangement, and lengths of helices can be freely designed. We further enabled building yet more sophisticated shapes that also twist and bend in desired ways (3). Importantly, DNA origami retains spatial registry over each of thousands of DNA bases that are installed in a constructed shape. These methods thus afford truly unique positional control on the nanoscale. Our current efforts are now centered around taking advantage of this positional control in the form of nanoscale "gadgets" for applications in the molecular biosciences.

(1) PWK Rothemund: NATURE 2006

(2) SM Douglas, H Dietz, T Liedl, B Hogberg, F Graf, W Shih: NATURE 2009

(3) H Dietz, SM Douglas, W Shih: SCIENCE 2009