Regensburg 2010 – scientific programme
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BP: Fachverband Biologische Physik
BP 3: DNA, RNA and Associated Enzymes
BP 3.8: Talk
Monday, March 22, 2010, 16:15–16:30, H43
Orientation Defined Stretching and Immobilization of DNA by AC Electrokinetics — •Venkatesh Alagarswamy Govindaraj1, Simone Herth1, Anke Becker1,2, and Günter Reiss1 — 1Thin Films & Physics of Nanostructures, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld. — 2Molecular Genetics, Institute for Biology III, Albert-Ludwigs-Universität Freiburg, Schänzlestr. 1, 79104 Freiburg.
DNA based single molecule studies, nano-electronics and nano-cargos require a precise placement of DNA in an orientation defined manner. Until now there is a lack of orientation defined stretching and immobilization of DNA for gaps smaller than several micrometers. However, this can be realized by designing bi-functionalized DNA with thiol at one end and (3-Aminopropyl) triethoxy silane on the other end, which specifically bind to gold and SiO2 layer after or during stretching. The electrode assembly consists of platinum as electrode material for applying the AC voltage and islands of gold and silicon dioxide fabricated at a distance of about 500-800 nm. The orientation defined stretching and covalent fixing of DNA was carried out at different frequency ranges of the applied electric field and observed after metallization of DNA by palladium ions in a Field Emission Scanning Electron Microscopy (FESEM).