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BP: Fachverband Biologische Physik

BP 32: Posters: Physics of Cells

BP 32.15: Poster

Donnerstag, 25. März 2010, 17:15–20:00, Poster B1

Theoretical and experimental studies of Protein Kinase C membrane translocation processes — •Martin Peglow¹, Mike Bonny¹, Heiko Rieger¹, Karsten Kruse¹, and Lars Kaestner² — ¹Theoretische Physik, Universität des Saarlandes, Campus, 66123 Saarbrücken — ²Institut für Molekulare Zellbiologie, Universitätsklinikum des Saarlandes, 66421 Homburg

Protein Kinase C α (PKCα) is a versatile key for decoding the cellular calcium toolkit. Once activated by cytosolic Ca²⁺ ions PKCα translocates to the plasma membrane and creates local patterns with limited spatial spread (< 4µm), the so-called local translocation events (LTEs). Two populations of LTEs exist, namely short lived events with lifetimes of 500-1500 ms and long lasting events with duration up to 10 seconds, which markedly exceeds the duration of the underlying calcium signals [1]. If we incorporate a possible interaction between membrane bound PKCα in our stochastic three-dimensional reaction-diffusion model, we can explain both LTE populations. In addition to our computer simulations, we perform fluorescence resonance energy transfer (FRET) measurements to give evidence for our assumption of a so far unkown interaction in between membran bound PKCs molecules.

[1] Gregor Reither, Michael Schaefer, Peter Lipp, Journal of Cell Biology, 174, 521-533 (2006)