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BP: Fachverband Biologische Physik
BP 32: Posters: Physics of Cells
BP 32.21: Poster
Donnerstag, 25. März 2010, 17:15–20:00, Poster B1
4D-Tracking of pathogens by Digital In-line Holographic Microscopy — •Sebastian Weiße1, Matthias Heydt1, Niko Heddergott2, Markus Engstler2, Michael Grunze1,3, and Axel Rosenhahn1,4 — 1APC, University of Heidelberg — 2Zoology I ,University of Würzburg — 3ITG, Karlsruhe Institute of Technology — 4IFG, Karlsruhe Institute of Technology
Digital Holographic Microscopy (DHM) using the in-line geometry is based on the original idea of Gabor's `new microscopic principle'. An interference pattern containing the three dimensional information of the object encoded in phase and amplitude is recorded. Using computers, real space information about the object can be restored from these holograms applying a reconstruction algorithm. We built a portable, temperature-controlled holographic microscope to study the motion patterns of pathogenic microorganisms such as the blood parasite Trypanosoma brucei, the causative agent of African sleeping sickness under physiological conditions. The directed self-propulsion of Trypanosomes in the bloodstream of a mammalian host is essential for the clearing of immunglobulins from the parasite's cell surface by hydrodynamic drag force. This mechanism is one of the parasite's strategies to evade the host's immune system and thus directly linked to pathogenesis. So far the motility studies on this uniflagellated microorganism have only been carried out using standard 2D microscopy techniques. In our system parasites were tracked at varying temperatures and viscosities with high spatial and temporal resolution and the obtained 3D motion patterns statistically analyzed.