Regensburg 2010 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 32: Posters: Physics of Cells

BP 32.24: Poster

Donnerstag, 25. März 2010, 17:15–20:00, Poster B1

Interaction between nanoparticles and living cells with force spectroscopy — •Sebastian Zünkeler, Daniel Wesner, Katja Tönsing, and Dario Anselmetti — Experimental Biophysics & Applied Nanoscience, Faculty of Physics, Bielefeld University

Nanotechnology is regarded as a key technology of the 21st century and nanotechnological systems are already used in many applications. However, the interaction between nanoparticles (NP) and living cells is not yet fully understood in the context of toxicology and need therefore additional characterization. To analyze these interactions with AFM force spectroscopy we have used an epoxide resin as adhesive for different metal oxide NP and attach aggregates which consist of primary particles to AFM-cantilevers. The characterization of the tip is possible with electron microscopy and the use of AFM and an inverse grid. We have shown that the used NP bind most likely unspecific to RLE-6TN rat lung cells. The number of rupture events increase with the contact time between tip and cell membrane and the measured rupture forces in the range of 50 pN depend mainly on micromechanical membrane properties.