Regensburg 2010 – scientific programme

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BP: Fachverband Biologische Physik

BP 32: Posters: Physics of Cells

BP 32.6: Poster

Thursday, March 25, 2010, 17:15–20:00, Poster B1

The automated Microfluidic Optical Stretcher — •Roland Stange, Tobias Kiesling, Bernd Kohlstrunk, and Josef A. Käs — University of Leipzig, Institute of Experimental Physics I, Linnestr. 5, 04103 Leipzig

Measuring the deformability of biological cells can be done in different ways. The most accurate one is the optical deformability measurement with the microfluidic optical stretcher. Due to the optical differences of each single living cell the scattering of the result has to be compensated with large numbers of measurements. According to the fact that the optical stretcher is operating with suspended cells in microfluidic channels it is complicated to handle by hand and time consuming to get precise and reliable data.

To improve the way of measuring the optical deformability we fully automated the optical stretcher by using a Labview program to control the flow pumps, the lasers and the camera. The produced data is then automatically evaluated by a Matlab program which finds out the deformation values from the images by an edge detection process. Furthermore the computer controlled microfluidic and photo lithographic produced measure chambers allow us to get different parameters out of the cell and sort them after measurement in a precisely controllable way.