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BP: Fachverband Biologische Physik
BP 33: Posters: Neurobiophysics
BP 33.6: Poster
Donnerstag, 25. März 2010, 17:15–20:00, Poster B1
Critical micellar concentration (CMC) dependence of pluronic effects on neuronal cells in culture — •Vicente D. Samith1,2,3, María J. Retamal2, Ignacio Vergara2, Esteban Ramos-Moore2, Ulrich G. Volkmann2, and Ricardo B. Maccioni1,4 — 1Laboratory of Cellular and Molecular Neurosciences, Faculty of Sciences, Universidad de Chile, Santiago de Chile — 2Dept. of Physics, P. Universidad Católica de Chile, Santiago de Chile — 3Dept. of Chemistry, Universidad Andrés Bello, Santiago de Chile — 4International Center for Biomedicine (ICC), Santiago de Chile
We are evaluating triblock copolymers, referred to as pluronics, for delivery of anti-inflammatory drugs that normally do not cross the blood-brain barrier. We studied the cytotoxicity of pluronic F68 in human neuroblastoma cells in culture, and analyzed physicochemical parameters of this type of polymeric matrixes such as the critical micellar concentration (CMC). Atomic force microscopy (AFM) was used to investigate the morphological changes in the lipid monolayer as a function F68 concentrations from 0.5 x 10−4 M to 10 x 10−4 M, adsorbed on a solid substrate (hydrophilic silicon). We observe a gradual change in the morphology of the polymer, from a ’dendritic’ to a supramolecular structure (clusters). The analysis of morphological changes of F68 is complemented by measurements of the percentage of film coverage on the silicon substrate as a function of the molar concentration of F68.
This work is supported by MECESUP, FSM 0605 and FONDECYT 1060628 (UGV) and 1080254 (RBM).