Regensburg 2010 – scientific programme

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BP: Fachverband Biologische Physik

BP 34: Posters: New Technologies

BP 34.8: Poster

Thursday, March 25, 2010, 17:15–20:00, Poster B2

Magnetic Tweezers Setup for Single Molecule Experiments — •Carolin Rademacher, Sebastian Horstmeier, Christoph Pelargus, Andy Sischka, and Dario Anselmetti — University of Bielefeld, Department of Physics, Experimental Biophysics and Applied Nanosciences

Besides atomic force microscopy and optical tweezers magnetic tweezers are a powerful tool for micromanipulations and single molecule force spectroscopy. We introduce a magnetic tweezers setup, that is based on a multipole-alignment operating with electromagnets to accomplish drag- and rotation-experiments with individual magnetic beads and allow operation at low mechanical vibrations. Since a wide range of applications is wanted, the pole pieces of the setup are manufactured through contact-lithography and electro-deposition [1], so that alterations are easy to accomplish in form and size. In the future we want to apply the magnetic tweezers to study rotating motor proteins under in vitro conditions. Here we present and discuss our setup and show the first data of our calibration experiments.

[1] A. H. B. de Vries, B. E. Krenn, R. van Driel, and J. S. Kanger. Patterned Electroplating of Micrometer scale Magnetic Structures on glass substrate. Journal of Microelectromechanical Systems (J-MEMS), 13:391 - 395, 2004.